網頁2012年12月28日 · We introduce a chromatography method for purification of large proteins and viruses that works by capturing them at a non-reactive hydrophilic surface by their … 網頁2013年3月22日 · We describe a new variant of anion exchange chromatography in columns packed with porous particles that embody charged low-density polymer zones supported by a higher density polymer skeleton. IgG defies the norms of anion exchange and is excluded to the void volume at pH 3–10 and 0–4 M NaCl. Void exclusion also occurs with Fab, F …

Principles and applications of steric exclusion chromatography

網頁2012年12月28日 · We refer to the technique as steric exclusion chromatography. Hydroxyl-substituted polymethacrylate monoliths provide a hydrophilic surface and support … 網頁2008年3月2日 · Purification of IgM Monoclonal Antibodies. Published on: March 2, 2008. Pete Gagnon, Frank Hensel, Richard Richieri. BioPharm International, BioPharm … office depot laser paper

BioPharm

網頁Steric exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of steric exclusion separations to be meaningful, … 網頁2012年11月6日 · IgM capacity was greater than 60 mg/mL. 95% of host cell proteins were eliminated from IgM produced in protein-free media, and mass recovery was up to 90%. … 網頁2014年1月10日 · We achieved exceptionally high capacity capture of monoclonal IgG by adding 200 nm starch-coated magnetic particles as nucleation centers, adding … my christ mychart