WebJan 1, 2012 · Sleeping Beauty (SB) was the first transposon ever shown capable of gene transfer in vertebrate cells, and recent results confirm that SB supports a full spectrum of … WebThe Sleeping Beauty (SB) transposon is a non-viral integrating system with proven efficacy for gene transfer and functional genomics. To optimize the SB transposon machinery, a transcriptionally regulated hyperactive transposase (SB100X) and …
Frontiers The Integration Preference of Sleeping Beauty …
WebThe Sleeping Beauty transposon system is a DNA transfer method that employs a transposase enzyme to cut and paste donor DNA flanked by Sleeping Beauty-specific inverted repeats/direct repeats (IR/DRs) into chromosomal DNA (1). This system allows … WebMar 10, 2024 · Recently, we proved that Sleeping Beauty (SB) transposon integrates into non-TA sites at a lower frequency. Here, we performed a further study on the non-TA integration of SB and showed that (1) SB can integrate into non-TA sites in HEK293T cells as well as in mouse cell lines; (2) Both the hyperactive transposase SB100X and the … top rated nfl players at each position
Frontiers An Efficient Electroporation Protocol for the Genetic ...
WebDec 13, 2024 · The Sleeping Beauty ( SB) transposon system is a powerful and unbiased genetic editing tool that can be used for rapid screening of candidate liver cancer driver genes. WebSep 8, 2011 · We describe an experimental approach for generating mutant alleles in rat spermatogonial stem cells (SSCs) using Sleeping Beauty (SB) transposon-mediated insertional mutagenesis. The protocol is based on mobilization of mutagenic gene-trap transposons from transfected plasmid vectors into the genomes of cultured stem cells. WebApr 15, 2024 · A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system. Lingli He, 1,4,* Wentao Yu, 1 Yi Lu, 1 Wenxiang Zhang, 1 Jinjin Xu, 1 and Lei Zhang 1,2,3,5,** ... The following protocol combines a genetically engineered mouse model (GEMM) with a sleeping beauty system to establish an in vivo liver ... top rated nfl quarterbacks 2019