Mouse ovariectomy protocol
Nettet13. aug. 2024 · Figure 1. Mouse treatment scheme. At weaning (age, 28 days), female apoE-/-C57BL/6J mice were anesthetized with avertin (tribromoethanol; 200 mg/kg; … NettetGeneration Mouse Artificial Decidualization Model With Ovariectomy. JoVE publishes peer-reviewed scientific video protocols to accelerate biological, medical, chemical and physical research. Watch our scientific video articles. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research, scientific …
Mouse ovariectomy protocol
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NettetWe report an efficient method of ovariectomy and compared this method with the two other operative methods of ovariectomy (i.e., midline dorsal skin incision and double dorsolateral approach, which are used commonly for inducing experimental osteoporosis. Female Wistar rats, 12 weeks old, were divided into three groups. Nettet1. mai 2024 · Upon recovery from surgeries and training (~6 weeks), all mice underwent the EHS protocol in an environmental chamber set at 37.5℃ and 40% relative humidity. Mice exercised on a running wheel ...
NettetIn the present study, we first developed a Laser Doppler Perfusion Measurement (LDPM) protocol in mouse tibia, which we validated with ex vivo and in vivo experiments. Then we carried out a study associating both structural (vascular quantitative histomorphometry) and functional (LDPM) approaches. Nettet8. feb. 2024 · 3.2.4 Operative Technique for Orchiectomy 1. Place the anesthetized animal on the operating table on its back with the tail toward you. 2. Make a single incision on the ventral side of the scrotum (0.5 cm for mice and 1.0 cm for rat) penetrating the skin using sterile scalpel ( see Fig. 2 a, b). Fig. 2 Illustration of orchiectomy in the rat.
Nettet1. jun. 2024 · This scanning protocol induced 256 mGy dose to the mouse and was found to have minimal effects on the bone properties evaluated in this study 40. A third-order polynomial beam hardening... Nettet8. feb. 2024 · This chapter describes the surgical procedures for ovariectomy and orchiectomy in mice and rats. In addition to providing technical details of the surgical …
NettetThe method presented here provides a detailed description of the ovariectomy surgical procedure (double dorsolateral incisions) in 8-week-old mice. For the surgery …
NettetIn ovariectomized animals, treatment with EM-800 decreased uterine and vaginal wt below the values observed in control ovariectomized mice while no significant change was observed on serum LH, thus indicating the lack of estrogenic activity of EM-800. untherapeuticalNettetThe Mclean Lab at the University of Michigan demonstrates how to perform a right ovariectomy. reclast used forNettet1. jan. 2003 · Otherwise, procedure of ovariectomy is very simple and takes 5-7 minutes for one mice to complete. It is also mandatory to administer antibiotics after surgery. reclawebreclawedNettet1. okt. 2012 · Mice were anesthetized using 2-3% isoflurane and subjected to sham surgery or ovariectomy by removing both ovaries, according to a previously published protocol [63]. After surgery, mice … reclawNettet29. jun. 2024 · In Asia, extracts of Lysimachia christinae have been used for liver or urinogenital system-related diseases in traditional medicine. In this study, we investigated the effects of the water extract of L. christinae (WELC) on receptor activator of nuclear factor-kappa Β ligand (RANKL)-induced osteoclastic differentiation of bone marrow … reclast what to expect afterwardsNettetNational Center for Biotechnology Information reclast wbc