WebMost mouse geneticists who work with Cre-lox mice probably are familiar with the FLP-FRT system. Like Cre, FLP is a tyrosine recombinase, but it originates from the yeast Saccharomyces cerevisiae, rather than from bacteriophage. In FLP-FRT, FLP is analogous to cre and FRT to loxP – FLP recognizes the FRT sites, and catalyzes recombination ... Web6 - 1 - FLP FRT 重组系统与 Mosaic 杂交实验（1）是【北京大学】遗传学实验的第29集视频，该合集共计37集，视频收藏或关注UP主，及时了解更多相关视频内容。
doi: 10.1007/978-1-0716-2201-8 4 - Springer
WebMay 1, 1998 · Recently, the use of the FLP/FRT system has allowed for the high-frequency production of mosaic animals for 95% of Drosophila genes. Cell markers have been engineered in this system to label ... WebJun 25, 2012 · Lee等发明了MARCM（mosaic analysis repressiblecell marker，嵌合体可抑制细 胞标记分析）技术 [63] ，这项技术依赖于FLP重组酶在 果蝇细胞有丝分裂期间，介 … dick\\u0027s sporting goods asheville nc
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WebMay 28, 1998 · A cassette was constructed that contains a GmR selectable marker next to the green fluorescent protein structural gene, with both markers being flanked by Flp recombinase target (FRT) sites. The FRT cassette was used to insertionally inactivate the cloned P. aeruginosa pabC gene encoding aminodeoxychorismate lyase. WebJan 11, 2007 · generate MARCM clones, as tubP–GAL80 will repress FLP expression. FRT site An FRTsite is a DNA sequence that, when inthe presence of FLP, will break and swap distal chromosome arms with the corresponding FRTsite. FRT sites are located close to the centromere on a chromosome arm of interest. A MARCM-ready stock will contain one … Webuse of flp/frt system and cell markers to perform mosaic analysis for existing mutations A general scheme for inducing mitotic clones using the site-specific recombinase FLP, its … city break flights