All strains used in this study are listed in Table 1. LB medium containing peptone (10 g/L), yeast extract (5 g/L), and NaCl (10 g/L), at pH 7.0 to 7.4, was used for the culture of E. coli DH5α. YPD medium containing glucose (20 g/L), peptone (20 g/L), and yeast extract powder (10 g/L) was used for the culture of C. … See more Based on the ctfat1p1 gene encoding the fatty acid transporter of C. tropicalis (NW_003020055.1), the upstream primer Fat1p F1 and the … See more After the single-knockout of the ctfat1p or ctpxa1p gene, the remaining copy was knocked out by constructing double-knockout fragment PFA6a-fat1p2 (5496 bp) or PFA6a … See more The preparation of C. tropicalis competent cells was performed according to the previously reported methods [17, 18]. ctfat1p1-kanr and ctpxa1p1-kanr were separately … See more Used C. tropicalis 1798 DNA as the template and Fat1p F2 and Fat1p R2 (Supplementary Table 1) as the primers for PCR amplification, a 3049-bp ctfat1p gene copy number was used to increase the ctfat1p gene … See more WebAug 1, 1972 · The better condition of cultivation for tetradecane 1,14-dicarboxylic acid (DC-16) production from n-hexadecane (n-C 16) by Candida cloacae MR-12 was …
Metabolic Engineering of Candida Tropicalis for the …
WebAug 1, 1992 · The engineered strain of the yeast, Candida tropicalis, is engineered for the efficient production of long–chain dicarboxylic acids and is effective for the selective terminal oxidation of both saturated and unsaturated linear aliphatic substrates with chain–lengths ranging from 12 carbons to 22 carbons. We have engineered an industrial … WebIn this study, the effect of fermentation substrate and its concentration on cell growth and dodecanedioic acid (3DA) production by wild type Candida tropicalis isolated from … diaphanous teal mystic lace fabric
Candida cloacae oxidation of long-chain fatty acids to dioic acids
WebAug 1, 2000 · Production of dioic acids. When grown in medium with glucose (5 g/l), C. cloacae will oxidize kerosene or its individual alkane components, from decane to hexadecane, to the corresponding dioic acids (Fig. 1). Decane is the least effective of these four substrates for dioic acid production, and shorter alkanes are toxic. WebOct 9, 2024 · There, the research focused on shorter chain lengths and varying production titers (0.5–174 g/l) were reported depending on the organism and production process. In Candida tropicalis, microbial production processes with genetically engineered strains were described for both C18 α,ω-dicarboxylic acids (DCAs) and C14 ω-HFAs (Craft et al. WebProduction of dodecanedioic acid (DDDA), a platform chemical used as raw material for various commodities and polymers, has been studied through a biological process. This … citicards cbna contact number